Studying Molecular Dynamics with High-Speed Atomic Force Microscopy
Andreas Kraus1,2, Andre Körnig1, and Heiko Haschke1
1JPK BioAFM, Bruker Nano GmbH, Am Studio 2D, 12489 Berlin, Germany
2Main presenter & contact (a.kraus@bruker.com)
Studying the self-assembly mechanism and activity dynamics of pore forming proteins is important for understanding of their biological significance in membrane degradation and developing appropriate therapeutic applications. Recent atomic force microscopy (AFM) technology developments have led to unprecedented imaging rates in fluid, setting new milestones in high-speed scanning capabilities. Bruker recently launched the fastest commercially available high-speed AFM (NanoRacer®) enabling real-time visualization of dynamic biological processes taking place on the sub-20-milisecond scale.
We will give an overview of correlative microscopy applications featuring fast imaging and advanced super-resolution optical setups. We will further introduce to the concept, theoretical background, high-speed application examples and experimentally demonstrate high-speed AFM operation.